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KMID : 0895820210310010059
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Journal of Oriental Rehabilitation Medicine
2021 Volume.31 No. 1 p.59 ~ p.79
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Genotoxicity Study of ChondroT
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Kim Sun-Gil
Kim Joo-Il
Kim Ji-Hoon
Yoon Chan-Suk
Jeong Ji-Won
Na Chang-Su
Kim Seon-Jong
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Abstract
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Objectives : This study was performed to observe the genotoxic effect of the ChondroT.
Methods :To evaluate the genotoxicity of ChondroT, an experiment of bacterial reverse mutation test, in vitro mammalian chromosomal aberration test and mammalian erythrocyte micronucleus test in mouse was conducted.
Results : TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies being greater than 2-fold of the respective negative control value. Both in -S9 mix and +S9 mix, the frequencies of aberration cells with structural aberration and numerical aberrations of chromosome were less than 5%. There was no increase of polychromatic erythrocyte with one or more micronuclei at any dose of test substance compared to the negative control group (p<0.05).
Conclusions : In TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies was greater than 2-fold of the respective negative control value, showing positive results. ChondroT was considered to be non-clastogenic to Chinese hamster lung (CHL/IU) cells under the present experimental condition. and ChondroT was determined not to induce an increased frequency of micronuclei in the bone marrow cells of male ICR mice under the present experimental condition.
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KEYWORD
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ChondroT, Mutagenicity tests, Chromosome aberrations, Micronucleus tests
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